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| Registros recuperados: 64 | |
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| Altangerel, Khukhuu; Sivakumar, Thillaiampalam; Inpankaew, Tawin; Jittapalapong, Sathaporn; Terkawi, Mohamad Alaa; Ueno, Akio; Xuan, Xuenan; Igarashi, Ikuo; Yokoyama, Naoaki; 玄, 学南; 五十嵐, 郁男; 横山, 直明. |
| Here we report on an epidemiological study regarding the molecular prevalence of different genotypes of Theileria orientalis present among domestic cattle and water buffalo populations bred in Thailand. A phylogenetic analysis based on the parasitic gene encoding a major piroplasm surface protein revealed the presence of 5 genotypes (Types 1, 3, 5, 7, and N-3) in cattle and 7 genotypes (Types 1, 3, 4, 5, 7, N-2, and N-3) in water buffaloes. Types 4, 7, and N-3 of T. orientalis were reported for the first time in water buffaloes. The previously reported C and Thai types from Thailand clustered as types 7 and 6, respectively, in the present analysis. Great similarities were observed among nucleotide sequences of isolates of the same genotype from cattle and... |
| Ano: 2011 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3592 |
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| Bannai, Hiroshi; Nishikawa, Yoshifumi; Seo, Jin-yong; Nakamura, Chinatsu; Zhang, Sofa; Kimata, Isao; Takashima, Yasuhiro; Li, Junyou; Igarashi, Ikuo; Xuan, Xuenan; 西川, 義文; 五十嵐, 郁男; 玄, 学南. |
| An enzyme-linked immunosorbent assay (ELISA) based on the recombinant p23 of Cryptosporidium parvum was established for the detection of antibodies against C. parvum in cattle. The sensitivity and specificity of the ELISA were evaluated with the standard indirect fluorescent antibody test (IFAT) using sporozoites as antigens. Of 77 bovine sera collected from China, 20 (26.0%) were identified as positive by the IFAT. The same samples were tested with the ELISA. The optical densities at 415 nm were compared to the IFAT results and statistically analyzed to designate a provisional cut-off point. As a result, the cut-off point was concluded to be 0.08, which was considered to be the best condition in the light of its sensitivity (80%) and specificity (73.7%).... |
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Palavras-chave: Cryptosporidium parvum; P23; ELISA; IFAT. |
| Ano: 2006 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/142 |
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| Bork, Sabine; Yokoyama, Naoaki; Okamura, Masashi; Igarashi, Ikuo; 横山, 直明; 五十嵐, 郁男. |
| Recently，we reported the in vitro growth inhibitory effects of the imidazole derivatives，clotrimazole(CLT) and ketoconazole (KC)，and the herbicide，clodinafop-propargyl(CP)，against bovine and equine Babesia parasites. Additionally，in the bovine species，several combined applications of the CLT，KC，and CP were reported to significantly increase the inhibitory effects. In this study，similar combination tests were carried out in order to confirm possible synergistic effects in the equine species，Babesia caballi and Babesia equi. Combinations of CLT/KC， CLT/CP，and CLT/KC/CP exerted a significantly enhanced growth inhibitory efficacy in B. caballi，in contrast to B. equi，where no synergistic effects were observed in any of the combinations. Our results suggest that... |
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Palavras-chave: In vitro; Babesia; Clotrimazole; Ketoconazole; Clodinafop-propargyl. |
| Ano: 2003 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/637 |
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| Claveria, Florencia G.; Omata, Yoshitaka; Takehara, Tokuma; Saito, Atsushi; Maki, Yoshiyuki; Igarashi, Ikuo; Suzuki, Naoyoshi. |
| Anti-Babesia rodhaini monoclonal antibodies (mAb), namely: 1-E7, 2-H2 and 3-B8, significantly suppressed the development of high parasitemia in BALB/c mice infected with B. rodhaini and all mAb-treated mice survived the infection. While, only monoclonal antibody 3-B8 showed some inhibitory effect against Babesia microti, with mice showing high parasitemia of 18.04 ± 2.69 %, at 9 days post-exposure. Westernblot analysis of B. rodhaini and B. microti parasite extract reacted with anti-B. rodhaini monoclonal antibodies showed cross-reactive bands of molecular weights 62 and 55 kilodaltons. Comparison of the antigenic components of B. rodhaini and B. microti using polyspecific sera revealed several shared or common parasite antigens of molecular weights 62,... |
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Palavras-chave: Monoclonal antibodies; Cross-reactivity; Babesia rodhaini; Babesia microti. |
| Ano: 1992 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/171 |
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| Fereig, Ragab M.; Mohamed, Samy G. A.; Mahmoud, Hassan Y. A. H.; AbouLaila, Mahmoud Rezk; Guswanto, Azirwan; Thu-Thuy Nguyen; Mohamed, Adel Elsayed Ahmed; Inoue, Noboru; Igarashi, Ikuo; Nishikawa, Yoshifumi. |
| Babesia bovis, B. bigemina, Trypanosoma evansi, and Anaplasma marginate infections cause serious diseases in cattle, and are primarily transmitted by arthropod vectors (ticks for B. bovis, B. bigemina, and A. marginate and various types of flies for T. evansi). In the last few years, there have been many reports of a high prevalence of certain protozoan infections in northern Egypt, but no accurate or adequate data are available for the southern regions. Therefore, in this study, we screened for evidence of such diseases in economically important cattle species using serum samples. The seroprevalence of protozoan infections in cattle was determined with enzyme-linked immunosorbent assays using species-specific diagnostic antigens. In a total of 301 cattle... |
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Palavras-chave: Babesia bovis; Babesia bigemina; Trypanosoma evansi; Anaplasma marginale; Cattle; Egypt. |
| Ano: 2017 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4579 |
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| Fujii, Kei; Yokoyama, Naoaki; Takabatake, Noriyuki; Suzuki, Hiroshi; Xuan, Xuenan; Igarashi, Ikuo; 横山, 直明; 鈴木, 宏志; 玄, 学南; 五十嵐, 郁男. |
| In hemoprotozoa，gene transfer technology provides an important tool to aid in the functional study of parasite genes. In this study，a transfer vector containing the enhanced green fluorescent protein (EGFP) gene laid between the Toxoplasma gondii GRAI promoter region and the GRA2 polyA signal region was constructed and transfected into the in vitro culture of Babesia boνis by the electroporation method.On the first and second days post-transfection，clear positive fluorescences were detected in some parasite bodies， indicating the successful expression of the EGFP gene controlled by the GRA-1 promoter in B. bovis. However， the positive parasites were not ubiquitous and finally disappeared until the forth day post-transfection. This is the first time that... |
| Ano: 2003 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/631 |
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| Fujii, Yasuhiro; Maki, Yoshiyuki; Ito, Masanari; Saito, Atsushi; Igarashi, Ikuo; Ono, Kenichiro; Itoh, Kikuji; Suzuki, Naoyoshi. |
| Large numbers of cyclophosphamide- or carrageenan-pretreated immunosuppressed adult ddY mice died within 10 days after inoculation with several species of opportunistic bacteria, including P. aeruginosa, K. pneumoniae, E. coli, S. aureus and methicillin resistant S. aureus. When immunosuppressed mice were administered bacteria in combination with two 100 μg/mouse intramuscular doses of a newly synthesized peptide, Obiopeptide-1 (OP-1), survival rates increased significantly. At 24 and 48 hours after intraperitoneal inoculation with P. aeruginosa, counts of viable organisms from the livers, spleens, lungs, hearts, and kidneys of mice that were administered cyclophosphamide in combination with OP-1 were significantly lower than counts from mice that were... |
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Palavras-chave: Immunoregulator; Synthesized peptide; Immunosuppression; Cyclophosphamide; Carrageenan; Ooiopeptide. |
| Ano: 1992 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/166 |
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| Fukumoto, Shinya; Sekine, Yukiko; Kimbita, Elikira; Huang, Xiaohong; Xuan, Xuenan; Inoue, Noboru; Yokoyama, Naoaki; Igarashi, Ikuo; Fujisaki, Kozo; Sugimoto, Chihiro; Nagasawa, Hideyuki; Mikami, Takeshi; Suzuki, Hiroshi; 福本, 晋也; 玄, 学南; 井上, 昇; 横山, 直明; 五十嵐, 郁男; 鈴木, 宏志. |
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Palavras-chave: Babesia gibsoni; Babesia canis; CDNA library; ELISA; P30 gene. |
| Ano: 2002 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/624 |
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| Fukumoto, Shinya; Tamaki, Yoh; Okamura, Masashi; Bannai, Hiroshi; Yokoyama, Natsuko; Suzuki, Tomoko; Igarashi, Ikuo; Suzuki, Hiroshi; Xuan, Xuenan. |
| A heterologous prime-boost immunization regime with priming DNA followed by recombinant vaccinia virus expressing relevant antigens has been shown to induce effective immune responses against several infectious pathogens. In this study, we constructed a recombinant plasmid and vaccinia virus, both of which expressed P50 of Babesia gibsoni, to investigate the immunogenicity and protective efficacy of a heterologous prime-boost immunization against canine babesiosis. The dogs immunized with the prime-boost regime developed a significantly high level of specific antibody against P50 when compared with the control groups, and the antibody level was strongly increased after a booster immunization with a recombinant vaccinia virus. The prime-boost immunization... |
| Ano: 2007 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/812 |
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| Goo, Youn-Kyoung; Terkawi, M. Alaa; Jia, Honglin; Aboge, G. Oluga; Ooka, Hideo; Kim, Suk; Igarashi, Ikuo; Nishikawa, Yoshifumi; Xuan, Xuenan. |
| The effect of artesunate, a water-soluble artemisinin derivative, against Babesia species, such as Babesia bovis, Babesia gibsoni, and Babesia microti was studied. Cultures of B. bovis and B. gibsoni were treated with 0.26 μM, 2.6 μM, 26 μM, and 260 μM artesunate, and the growth-inhibitory effect was shown in over 2.6 μM artesunate in day 4 and day 3 post-subculture for B. bovis and B. gibsoni, respectively, in dose-dependent manner. In vivo experiment for B. microti, strong inhibition effects were observed in mouse groups treated with over 1.0 mg/kg body weight of artesunate on day 9 and 10 post-infection. These results suggest that artesunate could be a potential drug for Babesia infection. |
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Palavras-chave: Artesunate; Babesia bovis; Babesia gibsoni; Babesia microti. |
| Ano: 2010 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2820 |
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| Ikadai, Hiromi; Sato, Masumi; Avarzed, Abgaandorjiin; Nagasawa, Hideyuki; Igarashi, Ikuo; Fujisaki, Kozo; Toyoda, Yutaka; Suzuki, Naoyoshi; 長澤, 秀行; 五十嵐, 郁男. |
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Palavras-chave: Purification; Merozoite; Babesia caballi. |
| Ano: 1997 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/272 |
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| Ishizaki, Takahiro; Sivakumar, Thillaiampalam; Hayashida, Kyoko; Tuvshintulga, Bumduuren; Igarashi, Ikuo; Yokoyama, Naoaki. |
| Babesia bovis is an apicomplexan hemoprotozoan that can invade bovine red blood cells (RBCs), where it multiplies asexually. RBC invasion assays using free viable merozoites are now routinely used to understand the invasion mechanism of B. bovis, and to evaluate the efficacy of chemicals and antibodies that potentially inhibit RBC invasion by the parasite. The application of high-voltage pulses (high-voltage electroporation), a commonly used method to isolate free merozoites from infected RBCs, reduces the viability of the merozoites. Recently, a cold treatment of B. bovis in vitro culture was found to induce an effective release of merozoites from the infected RBCs. In the present study, we incubated in vitro cultures of B. bovis in an ice bath to... |
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Palavras-chave: Babesia bovis; Cold treatment; Invasion-inhibition assay; Merozoite isolation; RBC invasion. |
| Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4488 |
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| Registros recuperados: 64 | |
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